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. 2018 Oct 31;4(10):eaat8131. doi: 10.1126/sciadv.aat8131

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Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

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Fig. 6 — (A) GFP-tagged Orai1 is present only at the cell membrane surface. (B and C) After delivery, Alexa Fluor 647–tagged STIM1 localizes to the cell surface, with a strong overlap with Orai1 (C), indicating that specific binding has occurred. (D) A line trace through the composite image (dashed line) shows colocalization of the STIM1 and Orai1 proteins at the membrane. (E to H) In Orai1-negative cells (E), the STIM1 is uniformly distributed throughout the cytosol. Scale bar, 5 μm. (H) The composite cross section shows that STIM1 does not localize to the cell membrane without the presence of Orai1.