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. 2018 Oct 25;27(21):1494–1506. doi: 10.1089/scd.2018.0124

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© Sofieke E. Klamer et al. 2018; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 1. — TGFBI expression in hematopoietic cells. (A) TGFBI mRNA expression was determined by qRT-PCR and calculated as fold change relative to GUS reference mRNA expression for different hematopoietic and BM niche cells. TGFBI mRNA is higher expressed in monocytes (Mono, n = 2) and MSCs (n = 2), than in immature CD34⁺ cells (HSPC BM, n = 3) and mature megakaryocytes (Mega, n = 2). (B) TGFBI mRNA expression in CD34⁺ subsets of the BM. MACS-isolated CD34⁺ HSPCs were incubated with antibodies and FACS sorted in the following progenitor subsets: the CD45^dimCD34⁺CD38⁺CD45RA⁻CD110⁺ fraction, representing MEPs (n = 2), the CD45^dimCD34⁺CD38⁺CD45RA⁻CD110⁻ fraction, representing CMPs (n = 3), and the CD45^dimCD34⁺CD38⁺CD45RA⁺CD110⁻ fraction, representing GMPs (n = 4). RNA was isolated from sorted cell fractions and TGFBI expression relative to reference gene GUS expression was analyzed by qRT-PCR. The TGFBI expression is significantly higher in GMP fractions compared to CMP fractions. *P < 0.05 (C) The relative TGFBI mRNA expression in CD34⁺ cells of various origin: isolated from CB (n = 4), MPB (n = 5), and BM (n = 4). The TGFBI expression is significantly higher in BM CD34⁺ cells compared to MPB-derived CD34⁺ cells. *P < 0.05. TGFBI, transforming growth factor beta-induced gene H3; qRT-PCR, quantitative reverse transcriptase PCR; BM, bone marrow; MSC, mesenchymal stromal cell; HSPC, hematopoietic stem and progenitor cell; MEP, megakaryocyte-erythrocyte progenitor; CMP, common myeloid progenitor; GMP, granulocyte-monocyte progenitor; CB, cord blood; MPB, mobilized peripheral blood; MACS, magnetic-activated cell sorting; FACS, fluorescence activated cell sorting.