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. 2018 Oct 25;27(21):1494–1506. doi: 10.1089/scd.2018.0124

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© Sofieke E. Klamer et al. 2018; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 2. — TGFBI expression in BM stromal cells. (A) TGFBI protein expression in MPB- and CB-derived HSPCs, primary MSCs, and L88-5 stromal cells was analyzed by western blot. Lysates containing equal cell numbers were loaded and a Histone-H3 antibody (17 kDa) was used as a loading control. TGFBI is detected at 70 kDa. (B) Endogenous localization of TGFBI (red), co-stained for F-actin (blue) in human primary MSCs after 24 h of adhesion to glass. TGFBI is deposited in the extracellular matrix and shows a footprint-like signature when MSCs are migrating. Scalebar indicates 20 μm. (C) Endogenous localization of TGFBI (red), co-stained for Glogin-97 (green) and F-actin (blue) in human primary MSCs. Zoomed regions show co-localization (yellow) of TGFBI with the golgi-associated protein golgin A1 (golgin-97). Scale bar indicates 50 μm.