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. 2018 Oct 25;27(21):1494–1506. doi: 10.1089/scd.2018.0124

FIG. 4.

FIG. 4.

TGFBI accelerates megakaryopoiesis and reduces net proliferation in granulocytic, erythrocytic, and monocytic differentiation. HSPCs with TGFBI overexpression and control cells were cultured toward different hematopoietic lineages for 14 days, quantified, and analyzed for the expression of specific surface markers by flow cytometry. (A, B) HSPCs were cultured toward megakaryocytes and analyzed for CD34 and CD41 surface expression. A representative experiment is shown in (A). The percentage of mature megakaryocytes (CD34-CD41+) was significantly increased upon TGFBI overexpression (n = 4). (C, D) HPSCs were cultured toward granulocytes and analyzed for CD11b and CD15 surface expression. Total granulocytic cell number, and cell surface markers CD11b and CD15 were significantly decreased upon TGFBI overexpression (n = 3). (E, F) HSPCs were cultured toward erythroblasts and analyzed for CD71 and CD235a surface expression (n = 3). Total erythrocytic cell numbers showed a downward trend upon TGFBI overexpression, no significant changes were observed in CD71 and CD235a cell surface expression. (G) HSPCs were cultured toward monocytes. Total monocytic cell numbers were significantly decreased upon TGFBI overexpression (n = 3). Shown are mean ± SEM. *P < 0.05, **P < 0.01, sh2/sh3 indicates that sh2 or sh3 was used in independent experiments.