Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Chemical compound, drug |
MyoVin-1 (Myo-1) | EMD Millipore |
Cat. No. 475984 |
|
| Chemical compound, drug |
Pentabrom opseudilin (PBP) |
FISHER SCIENTIFIC |
Cat. No. 501015859 |
|
| Chemical compound, drug |
EGTA-AM | FISHER SCIENTIFIC |
Cat. No. E1219 | |
| Chemical compound, drug |
SGC5 | VWR | Cat. No. 89410–772 | |
| Chemical compound, drug |
DL-AP5 Sodium salt |
Tocris | Cat. No. 3693 |
|
| Chemical compound, drug |
CNQX disodium salt |
Tocris | Cat. No. 1045 |
|
| Chemical compound, drug |
HEPES | Sigma | Cat. No. H4034 |
|
| Chemical compound, drug |
D-(+)-Glucose | Sigma | Cat. No. G 7021 |
|
| Chemical compound, drug |
Calcium chloride dihydrate |
Sigma | Cat. No. 223506 |
|
| Chemical compound, drug |
Magnesium chloride hexahydrate |
Sigma | Cat. No. M9272 |
|
| Chemical compound, drug |
Minimum Essential Media (MEM) - No Phenol Red |
thermofisher | Cat. No. 51200–038 |
|
| Chemical compound, drug |
Characterized Fetal Bovine Serum |
hyclone | Cat. No. SH30071.03 |
|
| Chemical compound, drug |
Penicillin- Streptomycin (5,000 U/mL) |
thermofisher | Cat. No. 15070063 |
|
| Chemical compound, drug |
N-2 Supplement (100X) |
thermofisher | Cat. No. 17502048 |
|
| Chemical compound, drug |
Donor Equine Serum |
hyclone | Cat. No. SH30074.03 |
|
| Chemical compound, drug |
Sodium Pyruvate (100 mM) |
thermofisher | Cat. No. 11360–070 | |
| Chemical compound, drug |
Neurobasal- A Medium |
thermofisher | Cat. No. 10888–022 | |
| Chemical compound, drug |
B-27 Supplement (50X), serum free |
thermofisher | Cat. No. 17504–044 | |
| Chemical compound, drug |
GlutaMAX Supplement |
thermofisher | Cat. No. 35050061 | |
| Chemical compound, drug |
Earle’s Balanced Salts |
sigmaaldrich | Cat. No. E3024 | |
| Chemical compound, drug | Corning Collagen I, Rat |
Fisher Scientific |
Cat. No. 354236 | |
| Chemical compound, drug |
Cover Glasses |
Fisher Scientific |
Cat. No. 12-545-80 | |
| Chemical compound, drug | Papain | Worthington Biochemical |
Cat. No. LS003126 | |
| Chemical compound, drug | PDL (poly-D-lysine) | BD Bios ciences |
Cat. No. 40210 | |
| Chemical compound, drug | Trypsin-EDTA (0.05%), phenol red | therm ofisher |
Cat. No. 25300–054 | |
| Biological sample (Rattus norvegicus, Female) |
Sprague-Dawley Timed-Pregnant rat, E15 pups |
Charles River |
Pups of both genders | |
| Software, algorithm |
MATLAB | MathWorks | RRID:SCR_001622 | |
| Software, algorithm |
u-track2.0 | Jaqaman, K., et al. (2008), Nat.Meth. 5, 695–702 |
Gaudenz Danuser Lab |
|
| Software, algorithm |
ImageJ | https://imagej.nih.gov/ij/ | RRID:SCR_003070 | |
| Software, algorithm |
Fiji | http://fiji.sc | RRID:SCR_002285 | |
| Software, algorithm |
inkscape | RRID:SCR_014479 | ||
| Other | VGluT1- pHluorin |
Drs. Robert Edwards and Susan Voglmaier (UCSF) |
Genetically encoded optical indicator of vesicle release and recycling |