Key resources table.
Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
---|---|---|---|---|
Gene (Homo sapiens) |
EAAT1WT | DNA2.0 synthetic | Uniprot P43003-1 | Codon optimized Homo sapiens |
Cell line (Homo sapiens) |
HEK293F | Thermo Fisher | R79007 | negative for mycoplasma |
Transfected construct (Homo sapiens) |
EAAT1CO | This paper | ||
Transfected construct (Homo sapiens) |
EAAT1COCO | This paper | ||
Transfected construct (Homo sapiens) |
EAAT1 ScaCO-TranDCOCO |
This paper | ||
Recombinant DNA reagent |
pCDNA3.1 | Invitrogen, doi: 10.1038/nature22064 |
||
Software, algorithm |
FELIX 4.1.2 | Photon Technology International/Horiba |
||
Software, algorithm |
DynamX 3.0 | Waters | ||
Software, algorithm |
HX-Express2 | http://www.hxms.com/HXExpress | ||
Software, algorithm |
Sigma Plot 12 | Sysat Inc. | ||
Software, algorithm |
Excel | Microsoft |