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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Curr Protoc Microbiol. 2018 Aug 20;51(1):e61. doi: 10.1002/cpmc.61

Figure 4. Gluing the fiber coverslip to a microscope slide.

Figure 4

A. When the coverslip has been fully removed from the genomic DNA solution, place a small drop of cyanoacrylate glue on a microscope slide, remove the coverslip from the molecular combing machine, and quickly place the coverslip on top of the glue, silane coating side up. Use as little glue as possible and work quickly as the glue rapidly dries.

B. For ease of scanning, it is important to have the coverslip as aligned with the microscope slide as possible. If the coverslip is skewed when placed on the glue, quickly adjust with forceps. As the cyanoacrylate glue spreads underneath the coverslip it rapidly dries, affording only a few seconds to make adjustments.