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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Curr Protoc Microbiol. 2018 Sep 25;51(1):e62. doi: 10.1002/cpmc.62

Figure 1.

Figure 1

ddPCR workflow: First a PCR mix containing target DNA along with primers and fluorescently labeled probes is partitioned into around 20,000 nanoliter sized droplets with the QX200 droplet generator. Then these droplets are cycled to an endpoint on a thermal cycler. Next the droplets are read with the QX200 Droplet Reader which sips each well, singulates the droplets and passes them by a two-color detection system. The data generated can finally be analyzed in the quantasoft software where thresholds should be set between the negative and positive droplets.