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. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Curr Protoc Microbiol. 2018 Sep 25;51(1):e62. doi: 10.1002/cpmc.62

Figure 8.

Figure 8

Trouble shooting example variance in mean fluorescence due to primer/probe mismatches: Primer/probe mismatches lead to a decrease in the mean fluorescence amplitude of positive droplets. A. Quantification schematic from a ddPCR run on patient plasma from three individuals compared to an RNA transcript control. B. The number of polymorphisms in the probe target region obtained by population sequencing and the corresponding mean amplitude of fluorescence in positive droplets obtained by ddPCR. C. Highlighter plot showing the population based and single genome sequences obtained from patient derived plasma from three individuals with the PCR region of interest compared to the primer sequences.