Skip to main content
. Author manuscript; available in PMC: 2019 Nov 1.
Published in final edited form as: Curr Protoc Microbiol. 2018 Sep 25;51(1):e62. doi: 10.1002/cpmc.62

Table 1.

ddPCR oligo sequences for DNA quantification

Target Primer Name Sequence Reference

CCR5 hCCR5-F1 5′-CCA GAA GAG CTG AGA CAT CCG-3′ Thomas, J.A. et al., 2006, Virology
hCCR5-R1 5′-GCC AAG CAG CTG AGA GGT TAC T-3′
FAM-hCCR5-P01 5′-/56-FAM/-TCC CCT ACA AGA AAC TCT CCC CGG-/3BHQ_1/-3′

LTR RU5-F 5′-CTT AAG CCT CAA TAA AGC TTG CC-3′
RU5-R 5′-GGA TCT CTA GTT ACC AGA GTC-3′
RU5-Probe 5′-/5HEX/-AGT AGT GTG TGC CCG TCT G-/ZEN//3IaBkFQ/-3′

gag HIV SCA 6F 5′-CAT GTT TTC AGC ATT ATC AGA AGG A-3′ Palmer, S. et al., 2003, J. Clin Microbiol
HIV SCA 84R 5′-TGC TTG ATG TCC CCC CAC T-3′
HIV SCA probe 32t 5′-/56-FAM/-CCA CCC CAC AAG ATT TAA ACA CCA TGC TAA-/ZEN//3IaBkFQ/-3′

tat Exon 1 TatRev1_F 5′-GCA TCC AGG AAG TCA GCC TA-3′
TatRev1_R 5′-CTT CGT CGC TGT CTC CGC-3′
TatRev1_probe 5′-/5HEX/-TC TTC CTG CCA TAG GAG ATG C-/3BHQ_1/-3′

tat/rev Exon 2 TatRev2_F 5′- GTG AAT AGA GTT AGG CAG GGA TA-3′
msRNA-R 5′-GTC TCT GTC TCT CTC TCC ACC-3′
msRNA Probe 5′-/56-FAM/-TCG GGC CTG TCG GGT CC-/3BHQ_1/-3′