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Duplexed ddPCR master mix to measure HIV DNA (HIV gag/RU5 copies)
| ddPCR Duplexed Master Mix
| ||
|---|---|---|
| Reagent | Final Concentration | Per Rxn (ul) |
|
| ||
| dH20 | 4.65 | |
| 2× BIO-RAD Supermix | 1× | 10 |
| 100um gag Forward | 750nm | 0.15 |
| 100um gag Reverse | 750nm | 0.15 |
| 100 ∝M gag FAM-probe | 200nM | 0.05 |
| 100∝M RU5 Forward | 750nm | 0.15 |
| 100∝M RU5 Reverse | 750nm | 0.15 |
| 100∝M RU5 HEX-probe | 200nM | 0.05 |
|
| ||
| Total | 15 | |
| Template | 5 | |
|
| ||
| Rxn Volume | 20 | |
