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. 2018 Sep 17;3(5):337–344. doi: 10.1002/lio2.180

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© 2018 The Authors. Laryngoscope Investigative Otolaryngology published by Wiley Periodicals, Inc. on behalf of The Triological Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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PRC2 components are active in basal cell cultures and normal adult mouse OE. (A) Protein extracts from cultures prepared from purified globose basal stem cells or from adult OE tissue analyzed by Western blot confirm expression of the PRC2 proteins SUZ12 and EZH2, and the H3K27me3 epigenetic mark, indicative of PRC2 enzymatic activity. CoxIV was used as a loading control. By immunohistochemistry, EZH2 (B) and SUZ12 (C) localize selectively to basal progenitor cell layers. Bar=10 μm; dashed line marks the basal lamina; nuclei counterstained with DAPI (blue). (D, E) Light microscopy image of basal cell–derived cultures demonstrates typical undifferentiated–appearing adherent islands; (E) area outlined by Box in D is shown at higher magnification; arrows mark rare cells with bipolar neuronal morphology arising on basal cell islands. Bar=20 μm in D.