Figure 6.

Polycomb repressive complexes are functional in OE cells. ChIP assays were performed on basal cell cultures. Q–PCRs were performed from ChIP DNA with primers to known PRC target genes (Hoxc10 or Pax2) or to a region of the beta actin gene which is not PRC–bound (Neg Ctl). Note that in input DNA, there is no enrichment of target genes compared to negative control; in contrast, PRC target genes are highly enriched in ChIP samples from anti–H3K27me3 or anti–RING1B experiments, consistent with functional Polycomb repressive complexes in OE cells.