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. 2018 Oct 25;5:152. doi: 10.3389/fcvm.2018.00152

Figure 2.

Figure 2

Functional analysis of cardiomyocyte-derived extracellular vesicles. (A) Representative images of flow cytometry analysis of non-conditioned media derived EVs stained (Sham) with CFSE, normoxia (Nx) and hypoxia (Hx) cardiomyocyte-derived extracellular vesicle (CM-EV) captured by fibroblasts (Fb) and endothelial cells (EC). (B) Flow cytometry quantification of fluorescence resulting from the incorporation of CFSE-labeled CM-EVs in Nx (black bars) and Hx (dashed bars) (n = 4, **P < 0.01). Data normalized to control condition. (C) Representative images of tube formation after 6 h of culture in the presence of Nx and Hx CM-EVs. (D) Quantification of total loops, loop length, total tubes and number of branching points from images taken after 6 h culture. Results are expressed in arbitrary units (n = 3, *P < 0.05). (E) Representative images of scratch assay after 24 h of culture in the presence of Nx and Hx CM-EVs. (F) Quantification of wound closure from images taken after 24 h culture normalized to initial wound area (n = 6, **P < 0.01).