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. Author manuscript; available in PMC: 2018 Oct 31.
Published in final edited form as: Adv Exp Med Biol. 2017;994:275–284. doi: 10.1007/978-3-319-55947-6_15

Fig. 15.3.

Fig. 15.3

HAE3 cell surface staining detected selective expression of the HAE3-cryptic glycan markers in human cancer cell lines. (a) Four tumor cell lines, T-47D, A549, PC3, and SKMEL-28, were stained with the C1 preparation of HAE3 (IgM) at 1:6 dilution or with an isotype control IgM,9.14.7 (5.0 μg/ml). (b) Seven breast cancer cell lines were stained with purified mAb HAE3(5.0 μg/ml) or 9.14.7 (5.0 μg/ml). These cell lines are T-47D, MCF-7, SK-BR-3, BT-549, Hs578T, MDA-MB-231, and MDA-MB-468. An R-PE-conjugated goat anti-mouse IgM antibody was applied to quantify the cell surface-captured IgM antibodies. Blue line: HAE3 stain; Red line:9.14.7 IgM isotype control (Adapted from Wang et al. 2015b)