Figure 3.

Sodium butyrate treatment regulates CD4⁺ T cell subsets (A). Splenic and PLNs' lymphocyte originated from sodium butyrate treated mice (n = 5), vehicle group (n = 5) and healthy mice (n = 3) were harvested for CD4, IL-4, IFN-γ, and IL-17A staining. The percentages of Th1, Th2, and Th17 cells were determined by flow cytometric analysis (B). The data are listed in the table as means ± SD of three independent experiment. The ratio of splenic Th1/Th2 and Th17 cell in PLNs was significantly higher than healthy mice, whereas sodium butyrate treatment restored unbalanced Th1/Th2 and regulated Th17 cells to normal level. *p < 0.05, **p < 0.01 vs. healthy group, ^#p < 0.05, ^##p < 0.01 vs. vehicle group.