Figure 4.

MiR-216b suppressed pancreatic cancer cell proliferation through targeting KRAS. A, C – The transfection efficiency of three KRAS siRNA transfected groups was determined. *P < 0.05, **p < 0.01, compared with control group. B, D, E – The expression of miR-216b in Panc-1 cells was significantly up-regulated, while the expression of KRAS was remarkably down-regulated after being transfected with KRAS-siRNA detected by qRT-PCR and western blot. **P < 0.01 compared with control group. F – The proliferation ability of Panc-1 cells after transfection with miR-216b mimics and KRAS-siRNA was significantly repressed, as observed by MTT assay. *P < 0.05, compared with control group