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. 2018 Oct 23;14(6):1308–1320. doi: 10.5114/aoms.2018.79004

Figure 5.

Figure 5

Figure 5

Overexpression of TRAF3IP2 reverses the anti-tumor effects of lncRNA UBE2CP3-001 shRNA. A – U87 cells were transfected with Lenti- GFP-TRAF3IP2, and the expression of TRAF3IP2 was analyzed by Western blot. B – The expression of MMP-9 was analyzed by Western blot after UBE2CP3-001 shRNA and Lenti-GFP-TRAF3IP2 transfection. C – The downregulation of TRAF3IP2 induced by UBE2CP3-001 shRNA rescued in the “UBE2CP3-001 shRNA + lenti-GFP-TRAF3IP2” cell line was measured by Western blot. D – The cell invasiveness of U87 cells upon transfection with UBE2CP3-001 shRNA and/or lenti-GFP-TRAF3IP2 was measured by the Transwell assay. E – The viability of U87 cells upon transfection with UBE2CP3-001 shRNA and/or lenti-GFPTRAF3IP2 was measured by the Cell Counting kit-8 assay. F – The cell apoptosis of U87 cells upon transfection with UBE2CP3-001 shRNA and/or Lenti-GFP-TRAF3IP2 was measured by flow cytometric analysis