Table 2.
List of main reagents used for immunohistochemistry
| Working dilution | Source | Catalogue no. | ||
|---|---|---|---|---|
| Primary antibody | Host | |||
| Anti‐GAD67 | Mouse | 1:200 | Abcam | Ab26116 |
| Anti‐parvalbumin | Rabbit | 1:500 | Swant | SV28 |
| Anti‐calretinin | Rabbit | 1:500 | Swant | 7699/3H |
| Anti‐calbindin D‐28k | Rabbit | 1:500 | Swant | CB38a |
| Anti‐choline acetyltransferase | Goat | 1:500 | Millipore | AB144P |
| Anti‐somatostatin | Rat | 1:100 | Millipore | MAB354 |
| Secondary antibody | Conjugated to | |||
|---|---|---|---|---|
| Anti‐mouse | Alexa488 | 1:200 | Dianova | 715‐545‐150 |
| Anti‐rabbit | CY3 | 1:400 | Dianova | 711‐165‐152 |
| Anti‐goat | CY3 | 1:300 | Dianova | 705‐165‐147 |
| Anti‐rat | CY3 | 1:400 | Dianova | 712‐165‐153 |
| Deep‐Red Nissl stain | — | 1:100 | Thermo Fisher Scientific | N21483 |
Sources: Abcam, Cambridge, UK; Swant, Marly, Switzerland; Millipore, Burlington, MA, USA; Dianova, Hamburg, Germany; Thermo Fisher Scientific, Carlsbad, CA, USA.