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. 2018 Oct 31;38(44):9446–9458. doi: 10.1523/JNEUROSCI.1676-18.2018

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Copyright © 2018 the authors 0270-6474/18/389446-13$15.00/0

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Figure 3. — BF cholinergic neurons are activated during fear conditioning. A, Transmitted light image showing regions profiled in B and C at bregma at 1.34 mm. EP, Entopeduncular nucleus; ic, internal capsule; GP, globus pallidus. B, AAV-TRE-mCherry-2a-tTA* injected in the nucleus basalis area of a ChAT-tauGFP × cFos-tTA mouse. Doxycycline transiently removed from the diet opens a labeling window during fear conditioning. White dotted inset delineates region magnified in C. C, Activated cholinergic neurons coexpress mCherry (red) and GFP (green) (yellow arrows). Cholinergic neurons are labeled by GFP. All neurons activated during fear conditioning are labeled by mCherry.