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. 2018 Oct 31;38(44):9514–9526. doi: 10.1523/JNEUROSCI.2911-17.2018

Figure 1.

Figure 1.

CREST expression. A, Mouse atlas image illustrates the region of the NAc where the immunohistochemistry staining image was taken. B, Immunoreactivity for CREST protein expression and its overlap with DAPI and NeuroTrace in NAc core is shown. CREST protein expression overlapping with DAPI and NeuroTrace staining. Scale bars: (in Merged) 7 μm; enlarged inset, 70 μm. C, A schematic of the experimental design showing that data were collected from mice killed after either saline or 5, 10, or 20 mg/kg cocaine-HCl conditioning in a single cocaine-CPP trial (see Materials and Methods). D, Gene expression was analyzed from tissue punches collected from the NAc. No changes in CREST mRNA after CPP conditioning with 5, 10, or 20 mg/kg cocaine-HCl relative to conditioning with saline were observed. E, Protein expression from tissue punches collected from the NAc of the same animals was quantified using Western blot. No changes in CREST protein after CPP conditioning with 5, 10, or 20 mg/kg cocaine-HCl relative to conditioning with saline were found. For CREST mRNA expression experiment, n = 7 for Saline, n = 7 for 5 mg/kg cocaine-HCl, n = 6 for 10 mg/kg cocaine-HCl, and n = 6 for 20 mg/kg cocaine-HCl. For CREST protein expression experiment, n = 10 for Saline, n = 7 for 5 mg/kg cocaine-HCl, n = 6 for 10 mg/kg cocaine-HCl, and n = 9 for 20 mg/kg cocaine-HCl.