Figure 2.

Anti-CREST siRNA in the NAc impairs cocaine CPP. A, Quantified Western blot data showing CREST protein levels following intra-NAc core siRNA infusion. siRNA anti-CREST in the NAc core results in a significant knockdown of CREST protein 2–6 d following infusion. B, Schematic of the cocaine CPP procedure. C, Cocaine CPP expression indicated by mean PS (CS+ − CS−) ± SEM. At 5 mg/kg cocaine-HCl training dose, siRNA anti-CREST mice exhibited a trend toward significantly attenuated CPP PSs compared with Control siRNA. D, At 5 mg/kg cocaine-HCl training dose, anti-CREST siRNA animals showed similar locomotion compared with nontargeting siRNA. For time course experiment, n = 3–4 per hemisphere per day. For cocaine CPP experiment, n = 9 for Control siRNA and n = 12 for anti-CREST siRNA. ****p < 0.0001.