Figure 6.

Effects of compound 5 on NF-κB/DNA interactions. (A) Compound 5 was pre-incubated for 20 min at the indicated concentrations with NF-κB p50 (Active Motif, cat.31101) and then a further 20 min incubation period was performed after adding the ³²P-labelled NF-κB probe. Then the complexes were separated by polyacrylamide gel electrophoresis. (B) Representative data showing the effects of trimethylangelicin (TMA) on the generation of NF-κB/DNA complexes (upper part of the panel). In the lower part of the panel the comparison of the effects of compound 5, TMA and corilagin is shown. (C) The same protocol described for panel A was followed, however, in this case, compound 5 was first incubated for 20 min with the ³²P-labelled NF-κB probe, after which the NF-κB p50 protein was added. (D) The effects of compound 5 were not dependent from DMSO, added in the EMSA reactions at a concentration corresponding to those of compound 5 incubations.