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. 2018 Sep 20;7(10):144. doi: 10.3390/cells7100144

Figure 4.

Figure 4

Effects of Intralipid® (lipid emulsion, LE) on the doxorubicin-induced reactive oxygen species (ROS) levels in H9c2 rat cardiomyoblasts. Cellular ROS were measured by flow cytometry after staining with dichlorodihydrofluorescein diacetate (DCFH-DA) following treatment with doxorubicin (10−5 M) alone for 1 h, pretreatment with LE (0.25%) or α-linolenic acid (10−6 M) for 1 h followed by doxorubicin (10−5 M) for 1 h, or treatment with LE (0.25%) or α-linolenic acid (10−6 M) alone for 2 h. (A,B) Effect of LE on ROS levels compared with the control. (C) Effect of α-linolenic acid on ROS levels compared with the control. (B,C) Data are presented as the mean ± SD (N = 3). * p< 0.001 versus control. † p < 0.001 versus doxorubicin alone.