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. 2018 Sep 28;10(10):362. doi: 10.3390/cancers10100362

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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MGMT promoter methylation status. (A) DNA was isolated from A2058 cells and several derived clones, and analyzed for methylation status of the MGMT promoter. Reference DNA from RKO and HCT8 colorectal carcinoma cell lines was included as negative (neg. ctr.) and positive controls (pos. ctr.), respectively. PMR: percentage of methylated reference. (B) A2058 cells were treated (Tx) with 1, 2, 3, or 4 cycles of 5-AzaC. Thereafter, cell lysates were prepared and analyzed for MGMT protein levels by WB. Actin was used as the loading control. Cell lysate labeled 4* was from cells that were cultured for an additional 4 weeks after 4 cycles of 5-AzaC treatment.