Figure 4.

EB-3D impairs MDA-MB-231 motility and invasiveness. (A) Representative images of wound closure at the beginning and end of the scratch experiment, 10× magnification. (B) Bar graphs showing the relative quantification of the distance between scratch edges. Confluent MDA-MB-231 monolayer was scratched and treated with EB-3D at the indicated concentrations and monitored at 6, 24, and 48 h. (C) Relative quantification of BME-based invasion assays performed with MDA-MB-231 pretreated with EB-3D for 24 h at the indicated doses. Data are represented as mean ± SEM of four independent experiments. (D) Relative mRNA expression levels of EMT-related genes assessed in MDA-MB-231 cells treated with 1 μM of EB-3D by qRT-PCR. Data were normalized by the expression levels of the housekeeping gene GUS and expressed as a fold change relative to untreated cells (DMSO) using the 2-ΔΔCt method. Data are represented as mean ± SEM of three independent experiments. Statistical significance was determined using Student’s t-test or ANOVA depending on the type of data. For multiple test comparison, Newman–Keuls corrections was applied. Asterisks indicate a significant difference between the treated and the control group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.