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. 2018 Sep 15;3(Suppl Suppl 1):53–68. doi: 10.1002/epi4.12254

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© 2018 The Authors. Epilepsia Open published by Wiley Periodicals Inc. on behalf of International League Against Epilepsy.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Hypothetical dose‐response curve calculation for Compound X by the Probit method. The ED50 is calculated to be 33.46 mg/kg [95% confidence intervals 25.42–43.03]. The black line indicates the calculated regression line and black dots represent actual data points for each treatment group. Line slope of 6.32 and standard error of 2.08. For an accurate ED50 calculation, the 95% confidence intervals should fall within the dose range tested.