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. 2018 Oct 15;12(11):1856–1870. doi: 10.1002/1878-0261.12384

Figure 2.

Figure 2

Metformin reduces MYC protein expression in CRC cells. (A) Metformin increases Wnt activity as evaluated by TOPFLASH luciferase reporter. (B) Metformin dramatically reduces MYC protein expression without affecting the MYC RNA level. (C) Metformin promotes glycolysis, as shown by acidic medium, and change of cell morphology into spindle‐like shape. (D) Metformin reduces MYC protein expression in both standard culture condition and glucose‐free condition. (E) Metformin diminishes MYC protein expression in colony formation cells. The luciferase data in (A) are 2 independent experiments (exp1 and exp2) in quadruplicate, and data represent means ± SD. The qRT‐PCR data in (B) are presented as the means ± SD (HCT116, n = 2; HT29, n = 4). Student's t‐test was used to assess significance. *P < 0.05.