Figure 6.

Metformin preferably represses the protein synthesis of cell cycle‐related genes. (A) Venn diagram of proteins that are downregulated by metformin (2 mm for 24 h) in four CRC cell lines identified with reverse‐phase protein array (RPPA). (B) Validation of the overlapping downregulated proteins by western blot. (C) Enrich analysis of the 16 downregulated proteins by RPPA revealed an enrichment of proteins that regulate cell cycle. (D) Metformin reduces the expression of p‐MNK1, concordant with the downregulated MNK1 by metformin in RPPA analysis. (E) A schematic diagram presenting the proposed mechanism of metformin effects.