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Saturation curves for determination of the dissociation constants (KD) of RNA aptamers specific to (A) HE: HE-08, HE-13, and HE-14 and to (B) CH: CH-09, CH-20, and CH-32. A control of immobilized biotin was used in both cases. KD values were calculated by nonlinear regression analysis based on the saturation curves.
