Figure 4.

Treatment of dermal fibroblasts with increasing concentrations of ivermectin results in the upregulation of GFAP as well as the development of an elongated morphology reminiscent of Schwann cells. (a) Dermal fibroblasts were treated with varying concentrations of ivermectin for 4 and 8 days, then subjected to qRT-PCR analysis of GFAP expression. (b) GFAP immunostaining demonstrates that dermal fibroblasts treated with relatively higher concentrations of ivermectin for 8 days results in an increase of GFAP expression as well as a change in morphology, which resembles a Schwann cell-like phenotype, scale bar: 100 μM. ***P ≤ 0.001; as determined by one-way ANOVA with post-hoc Tukey test. Error bars show mean ± SD.