TABLE 3.
Primers used in this study
| Name | Sequence from 5′ end | Descriptiona |
|---|---|---|
| P1 | AAGCCAAGAAAAACGTCGCCAACGTCGACATGAACATCAAAAAGTTTGCA | F sacB for pAK914 |
| P2 | GCTCGGTACCCGGGGATCCTCTAGAGGCCAATAGGATATCGGCATTT | R sacB for pAK914 |
| P3 | CGACTCTAGAGGATCCCCGGGTACCGTAGCTTCACGCTGCCGCAAG | F Pnpt for pAK920 |
| P4 | CCCGAATGTGCATGCGAAACGATCCTCATCCTGTC | R Pnpt for pAK920 |
| P5 | AGGATCGTTTCGCATGCACATTCGGGATATTTCTCTA | F strAB for pAK920 |
| P6 | TAATACGACTCACTATAGGGAATTCGCCCAGGGGATAGGAGAAGTC | R strAB for pAK920 |
| P7 | AAATGCCGATATCCTATTGGCCTCTAGAGAGATCGCGAAGCAGAGC | F kupM upstream for pAK941 |
| P8 | TGCGGCAGCGTGAAGCTACGGTACCGCCGTAATGCGTCAGAAAGT | R kupM upstream for pAK941 |
| P9 | CTTCTCCTATCCCCTGGGCGAATTCAGCCGGGTCATGGAAGTC | F kupM downstream for pAK941 |
| P10 | CGAGCTCGGTACCCGGGGATCCTCTAGAGGCCAGGGAATGGAGTTT | R kupM downstream for pAK941 |
| P11 | GGTACCGTAGCTTCACGCTGCCGCA | F Pnpt-strAB for pAK941 |
| P12 | GAATTCGCCCAGGGGATAGGAGAAGTCGCT | R Pnpt-strAB for pAK941 |
| P13 | GCCGATATCCTATTGGCCTCTAGAGCCTCCCAGATCGACCAGTC | F upp upstream for pAK1126 |
| P14 | CTATTTGGTGCCGGATCCCATGGACGCGCTCCTGGG | R upp upstream for pAK1126 |
| P15 | AGCGCGTCCATGGGATCCGGCACCAAATAGGGGG | F upp downstream for pAK1126 |
| P16 | CGACTCACTATAGGGAATTCGAGCTCGCCAGGCAGACGGCGGTG | R upp downstream for pAK1126 |
| P17 | GCCGATATCCTATTGGCCTCTAGAGAAGCTCGCCGAAAAGACC | F Pupp-upp for pAK1127 |
| P18 | CGACTCACTATAGGGAATTCGATGAAGGCGAACGAGGAAC | R Pupp-upp for pAK1127 |
| P19 | GCCCGCATTGAGGACGTG | To check upp deletion |
| P20 | CAGCGCCCCGAGCTTGCC | To check upp deletion |
| P21 | CGTCAGCAGGCAAACGG | To check kupM deletion |
| P22 | ACCGTTGTCTCCCATGTCTC | To check kupM deletion |
a
F, forward; R, reverse.