TABLE 4.
Primer sequences
| Function | Gene | Primer sequence (5′→3′)a | Size (bp) |
|---|---|---|---|
| Housekeeping | 16S rRNA | F: AGAGGATGACCAGCCACAC | 194 |
| R: CGGGTAACGTCAATGAGCAAAG | |||
| Membrane stress response | pspD | F: GGCAAAAGGTAAAGCCTGGT | 112 |
| R: CGGCGAGCAACTGATTTTAT | |||
| pspG | F: GTTTCGTTGCTGGGCATTAT | 101 | |
| R: AGTAACCACGGCAGCAACTT | |||
| Heat stress resistance | ibpB | F: CAAGGGTAATGCGGTAGTGG | 116 |
| R: TCAATGGATCGGTTTTGACA | |||
| Oxidative stress resistance | grxA | F: TCAGGCGTCCAGATTCTCTT | 143 |
| R: GTGCGGAAGGGATCACTAAA | |||
| soxS | F: ACGGCGTTGACGAATGTAAT | 107 | |
| R: ACATTGATGTCGTCGCAAAA | |||
| Iron transport | ECs0632 | F: GACTCAGGCGATGAAAGAGG | 112 |
| R: TTCAAAGGGAGTTGCGAGAT | |||
| feoA | F: CGCCAAAAACTGCTTTCTCT | 118 | |
| R: TGCGTAATACCAGGCTCACA |
a
F, forward; R, reverse.