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(A) Schematic map of the expression plasmid pJK3. Binding sites and the orientation of primers p352 and p353 are displayed as black arrows. (B) PCR-based confirmation of pJK3. A gradient PCR using biomass from the M. xanthus expression strain as the template was performed in order to amplify pJK3 with primers p352 and p353. The expected amplicon is marked with an arrow. Lane M, DNA ladder.
