TABLE 2.

Oligonucleotide primers^a used in this study

Primer	Gene	Nucleotide sequence
Quantitative RT-PCR
CYP505D1-f	CYP505D1	5′-CAAGTCCATTTGGGACGACT-3′
CYP505D1-r		5′-ACGAGACCAGGAGATGGAGA-3′
CYP505D2-f	CYP505D2	5′-ATGGAGAAGGCCAAGAACCT-3′
CYP505D2-r		5′-AGGAGGATGGACATGACGAC-3′
CYP505D3-f	CYP505D3	5′-ATGGAGAAGGCCAAGAACCT-3′
CYP505D3-r		5′-AGGAGGATGGACATGACGAC-3′
CYP505D4-f	CYP505D4	5′-ATGGAGAAGGCCAAGAACCT-3′
CYP505D4-r		5′-AGGAGGATGGACATGACGAC-3′
CYP505D5-f	CYP505D5	5′-ATGGAGAAGGCCAAGAACCT-3′
CYP505D5-r		5′-AGGAGGATGGACATGACGAC-3′
CYP505D6-f	CYP505D6	5′-CAGCTCCTCAAGTCCTACGG-3′
CYP505D6-r		5′-TCCTTCACAAGCTCCTCGAT-3′
CYP505D7-f	CYP505D7	5′-CATGGTCTGTGGTGTTGAGC-3′
CYP505D7-r		5′-CGAAGTTGTCGTCCTCCTTC-3′
ACT1-f	ACT1	5′-CCAAGGCTAACCGTGAGAAG-3′
ACT1-r		5′-CACCAGAGTCGAGCACGATA-3′
Cloning for recombinant protein production
CYP505D6-f	CYP505D6	5′-ATGACGTCTACCATTCCGACGCCGCCGTCCAT-3′
CYP505D6-r		5′-CTATTCGAAGATATCGGTAGCGAA-3′
CYP102A1-f	CYP102A1	5′-ATGACAATTAAAGAAATGCCTCAGCCAAAAAC-3′
CYP102A1-r		5′-TTACCCAGCCCACACGTCTTTTGC-3′
Site-directed mutagenesis
CYP505D6 V51Y-f	CYP505D6	5′-TACGTCAGCTCGGCGAAGCTCAT-3′
CYP505D6 V51Y-r		5′-GAGCAGCTTCCTCCCGAGAA-3′
CYP102A1 Y51V-f	CYP102A1	5′-GTCTTATCAAGTCAGCGTCTAAT-3′
CYP102A1 Y51V-r		5′-GCGCGTTACACGACCAGGCG-3′

^aGene-specific primers were designed based on genomic sequence data (http://genome.jgi.doe.gov/Phchr2/Phchr2.home.html).