TABLE 3.

Detailed information on the functional enzymes involved in MCCA formation

Abbreviation	Name	Gene	Definition	KO entry	EC no.	COG	MT		MG		MT/MG
							No. of hits	%	No. of hits	%
Reverse β oxidation
TLA	Thiolase	fadA	Acetyl-CoA acyltransferase	K00632	2.3.1.16	COG0183	78	0.0157	1,429	0.0487	0.32
		HADHB	Acetyl-CoA acyltransferase	K07509	2.3.1.16		1	0.0002	3	0.0001	1.96
KCR	Ketoacyl-CoA reductase	phbB	Acetoacetyl-CoA reductase	K00023	1.1.1.36	COG1028	36	0.0072	241	0.0082	0.88
HCD	Hydroxyacyl-CoA dehydratase	fadB	3-Hydroxybutyryl-CoA dehydrogenase	K00074	1.1.1.157	COG1250	1,404	0.2820	2,089	0.0713	3.96
		fadJ	3-Hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase	K01782	1.1.1.35, 4.2.1.17, 5.1.2.3	COG1250, COG1024	8	0.0016	201	0.0069	0.23
		fadN	3-Hydroxyacyl-CoA dehydrogenase	K07516	1.1.1.35	COG1250, COG1024	29	0.0058	1,338	0.0456	0.13
		HADH	3-Hydroxyacyl-CoA dehydrogenase	K00022	2.3.1.16		4	0.0008	29	0.0010	0.81
ECR	Enoyl-CoA reductase	ysiB, fadB	Enoyl-CoA hydratase	K13767	4.2.1.17	COG1024	14	0.0028	109	0.0037	0.76
		fadJ	3-Hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase/3-hydroxybutyryl-CoA epimerase	K01782	1.1.1.35, 4.2.1.17, 5.1.2.3	COG1250, COG1024	8	0.0016	201	0.0069	0.23
		paaF, echA	Enoyl-CoA hydratase	K01692	4.2.1.17	COG1024	85	0.0171	2,867	0.0978	0.17
		echA	Ech hydrogenase subunit A	K14086			6	0.0012	55	0.0019	0.64
		crt	3-Hydroxybutyryl-CoA dehydratase	K01715	4.2.1.17		716	0.1438	563	0.0192	7.49
TES	Thioesterase	yciA	Acyl-CoA thioesterase YciA	K10806	3.1.2.−	COG1607	7	0.0014	71	0.0024	0.58
Fatty acid biosynthesis
ACC	Acetyl-CoA carboxylase	accA	Acetyl-CoA carboxylase carboxyl transferase subunit alpha	K01962	6.4.1.2	COG0825	128	0.0257	1,284	0.0438	0.59
		accB	Acetyl-CoA carboxylase biotin carboxyl carrier protein	K02160		COG0511	52	0.0104	331	0.0113	0.93
		accC	Acetyl-CoA carboxylase, biotin carboxylase subunit	K01961	6.4.1.2, 6.3.4.14	COG0439	256	0.0514	3,059	0.1043	0.49
		accD	Acetyl-CoA carboxylase carboxyl transferase subunit beta	K01963	6.4.1.2	COG0777	94	0.0189	1,067	0.0364	0.52
MAT	Malonyltransferase	fas	Fatty acid synthase, bacteria type	K11533	2.3.1.−		3	0.0006	3	0.0001	5.89
		fabD	[Acyl-carrier-protein] S-malonyltransferase	K00645	2.3.1.39	COG0331	283	0.0568	1,436	0.0490	1.16
KAS	Ketoacyl-ACP synthase	fas	Fatty acid synthase, bacteria type	K11533	2.3.1.−		3	0.0006	3	0.0001	5.89
		fabB	3-Oxoacyl-[acyl carrier protein] synthase I	K00647	2.3.1.41	COG0304	20	0.0040	227	0.0077	0.52
		fabF	3-Oxoacyl-[acyl carrier protein] synthase II	K09458	2.3.1.179	COG0304	1,224	0.2458	3,931	0.1341	1.83
		fabH	3-Oxoacyl-[acyl carrier protein] synthase III	K00648	2.3.1.180	COG0332	617	0.1239	3,341	0.1140	1.09
KAR	Ketoacyl-ACP reductase	fas	Fatty acid synthase, bacteria type	K11533	2.3.1.−		3	0.0006	3	0.0001	5.89
		fabG	3-Oxoacyl-[acyl carrier protein] reductase	K00059	1.1.1.100	COG1028	1,003	0.2014	5,275	0.1799	1.12
HAD	Hydroxyacyl-ACP dehydratase	fas	Fatty acid synthase, bacteria type	K11533	2.3.1.−		3	0.0006	3	0.0001	5.89
		fadZ	3-Hydroxyacyl-[acyl carrier protein] dehydratase	K02372	4.2.1.59	COG0764	241	0.0484	1,007	0.0343	1.41
EAR	Enoyl-ACP reductase	fas	Fatty acid synthase, bacteria type	K11533	2.3.1.−		3	0.0006	3	0.0001	5.89
		fabI	Enoyl-[acyl carrier protein] reductase I	K00208	1.3.1.9, 1.3.1.10	COG0623	150	0.0301	777	0.0265	1.14
		fabK	Enoyl-[acyl carrier protein] reductase II	K02371	1.3.1.−	COG2070	163	0.0327	884	0.0302	1.09
		fabL	Enoyl-[acyl carrier protein] reductase III	K10780	1.3.1.−	COG1028	6	0.0012	216	0.0074	0.16