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. 2018 Sep 26;10(10):354. doi: 10.3390/cancers10100354

Figure 7.

Figure 7

Expression of signal proteins affected by C. coccineum: (A) MDA-MB-231 cells were cultured to subconfluency and treated with ethanol extract of C. coccineum (50 μg/mL). Total RNAs were harvested followed by measurement of Foxo3 expression by real-time PCR. Treatment with C. coccineum increased Foxo3 mRNA expression. (B) Cell lysate was also prepared from the cells treated with C. coccineum followed by Western blotting probing with antiFoxo3 antibody. Similarly, treatment with C. coccineum increased Foxo3 protein expression. (C) Expression of c-myc, pAKT, and LC3-B was also analyzed in the cell lysate. Treatment with C. coccineum decreased expression of these proteins. ** p < 0.01.