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. 2018 Sep 29;9(10):475. doi: 10.3390/genes9100475

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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The transmembrane helices, alignment, and phylogenetic tree analysis of HKT (high-affinity K⁺ transporter) amino acid sequences. (A) The transmembrane helices of PeHKT1;1 protein were predicted by TMHMN Server v.2.0. (B) The four conserved selectivity-filter-pore regions of HKT were aligned, and highlighted in yellow, using ClustalX 2.1 software. A line above the alignment was used to mark strongly conserved positions. Three characters (“*”, “:” and “.”) were used: “*” indicates positions which have a single, fully conserved residue. “:” and “.” indicates positions that have ‘strong’ and ‘weaker’ conserved residues, respectively. (C) Phylogenetic tree analysis of 14 HKTs from 12 different plant species by neighbor-joining method using MEGA v7.0 software with 1000 iterations bootstraps. HKT: high-affinity K⁺ transporter.

The transmembrane helices, alignment, and phylogenetic tree analysis of HKT (high-affinity K⁺ transporter) amino acid sequences. (A) The transmembrane helices of PeHKT1;1 protein were predicted by TMHMN Server v.2.0. (B) The four conserved selectivity-filter-pore regions of HKT were aligned, and highlighted in yellow, using ClustalX 2.1 software. A line above the alignment was used to mark strongly conserved positions. Three characters (“*”, “:” and “.”) were used: “*” indicates positions which have a single, fully conserved residue. “:” and “.” indicates positions that have ‘strong’ and ‘weaker’ conserved residues, respectively. (C) Phylogenetic tree analysis of 14 HKTs from 12 different plant species by neighbor-joining method using MEGA v7.0 software with 1000 iterations bootstraps. HKT: high-affinity K⁺ transporter.