Table 1.

Primers used in this study.

Primer ID	Forward (5′-3′)	Reverse (5′-3′)
3′RACE outer	ATCTATTGTCGATCTCTCCATC	TACCGTCGTTCCACTAGTGATTT
3′RACE inner	TGCCGAAAAAGCAACAGGAAGAGGTTGA	CGCGGATCCTCCACTAGTGATTTCACTATAGG
5′RACE outer	AGAACCCGACATTTCCGTATGC	CATGGCTACATGCTGACAGCCTA
5′RACE inner	GGTGAGAACAACAGGCACTGAACCAAAGAC	CGCGGATCCACAGCCTACTGATGATCAGTCGATG
PeHKT1;1-ORF	ATGAAGAGCTTTGCTAGT	CTAGGATAGCTTCCAAGCTTTACCA
SqRT-PCR	TCTTCGGCAACAGTTTCAAG	CCACACAAGCAAGGCTCTTA
qRT-PCR	GGCTATAGCTGCAAACGACA	AAGCCTTCCGAAGAGCATTA
Eflα	GGCAAGGAGAAGGTACACAT	CAATCACACGCTTGTCAATA
18SrRNA	TCAACTTTCGATGGTAGGATAGTG	CCGTGTCAGGATTGGGTAATTT
BP detection	ATGAAGAGCTTTGCTAGT	TAATACGACTCACTATAGGG’
LR detection	CGCACAATCCCACTATCCTT	CTAGGATAGCTTCCAAGCTTTACCA
Transgene detection	CGCACAATCCCACTATCCTT	CTAGGATAGCTTCCAAGCTTTACCA

RACE: rapid amplification of cDNA ends; ORF: open reading frames; SqRT-PCR: semi-quantitative reverse transcription PCR; qRT-PCR: quantitative real-time PCR; BP: BP clonase; LR: LR clonase; 18SrRNA: 18S ribosomal RNA.