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Quantitative proteomic workflow. HepG2.2.15 cells were treated with 100 ng/ml of IFN-λ3 and IFN-α2a and PBS for 24 h. Cell lysates of each group were digested and then labeled with different dimethyl reagents. After combining the 3 samples, these peptides were fractionated and then analyzed by LC-MS/MS.
