Fig. 9.

IFN-λ3 enhanced antigen processing/presentation. IFN-λ3 not only up-regulated HLA class I expression but it also increased the expression of other effector molecules involved in antigen processing and antigen presentation. Ubiquitinating enzymes that promote protein degradation were found to be up-regulated whereas deubiquitinating enzymes known to remove ubiquitin were found to decrease after IFN-λ3 treatment. Although all identified subunits of the constitutive proteasome cap were down-regulated, the 2 subunits of the immunoproteasome cap as well as some subunits of the proteasome core were elevated in expression. Several effector molecules involved in peptide loading on class I HLA as shown in red text were up-regulated with the exception of CALR, which was down-regulated as shown in green text. The italic red text represents proteins with increased expression levels that failed to reach statistical significance.