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Restoration of fucosylation and expression of MUC2 in HCT116 cells. Lectin blotting with AAL (A) and MUC2 qPCR analysis (B) of parental and GNAS mutant HCT116 cells, which have defective fucosylation because of deletion in GMDS gene, before and after restoration of fucosylation by transfection of wildtype GMDS containing vector. The values in B are means ±S.E. from three transfection experiments (n = 9). α-tubulin serves as a loading control in A.
