Fig. 4.

Peroxisomes are required for efficient ZIKV infection. A, Multiple ZIKV proteins interact with peroxisome and/or peroxisomal regulatory polypeptides. B, GFP-NS2A colocalizes with the peroxisome protein ABCD3/PMP70. C, GFP-NS2A expressed in wild type (WT) human fibroblasts localizes to peroxisomes but is mislocalized to the cytoplasm in PBD399 and PBD400 peroxisome-free cells. D, (top) Whole cell lysates from ZIKV-infected Vero cells were subjected to Western blotting, using antibodies directed against the indicated proteins. Quantitation of results (bottom) presented as bar graphs; *p < 0.05. E, Uninfected (Day 0) and ZIKV-infected Vero cells (3d post-infection), coimmunostained for ZIKV and PMP70. F, Quantitation of peroxisome density (peroxisomes/cell volume) in cells expressing GFP-NS2A *p < 0.05 or; G, infected with ZIKV; **p < 0.01. H, Viral titers (at indicated times post-ZIKV infection) were measured in the indicated cell lines (GM5756, wild type human fibroblasts); *p < 0.05; **p < 0.01.