Figure 1.

Y-box-binding protein 1 (YB-1) and Poly(A)-binding protein 1 (PABP1) co-localize and interact in stress granules (SGs) under stress conditions. (a) Confocal immunofluorescence of un-treated (control) HEK293T, and treated with 250 µM sodium arsenite (Na Ars) for 30’, 500 µM H₂O₂ for 1 h or subjected to heat shock at 45 °C for 1 h, stained with α-YB-1 (green) and α-PABP1 (red); yellow and white arrows indicate P-bodies and stress granules respectively; (b) (Upper panel) size and number (lower panel) of SGs after treatments compared to control; statistical analysis was performed using 1-way ANOVA followed by Dunnett’s multiple comparisons test. Levels of significance are indicated (*** p < 0.001, * p = 0.001, see also Supplementary Materials Table S2); (c) Co-immunoprecipitation of HEK293T total protein extracts treated (+) or not (−) with 250 µM Na Ars for 30’; extracts were immunoprecipitated for YB-1 and immunorevealed for PABP1. Input samples immunorevealed with α-PABP1 and α-YB-1 are shown. Each panel is assembled from cropped Western blotting images (see original Western blot file for the original images).