Figure 4.

Ch14.18/CHO-mediated ADCC and impact of HACA response. Induction of GD₂-specific ch14.18/CHO-mediated ADCC in HACA-negative (A) and HACA-positive pts (non-neutralizing (B) and neutralizing pts (C)) treated with the LTI regimen was analyzed in cycles 1, 3 and 5 on d15 (closed circles) and compared to the baseline cytotoxicity of the respective cycle (d1; open circles). ADCC was evaluated against the GD₂-positive NB cells LAN-1 as described in “Materials and Methods”. The circles represent pts evaluable for the analysis (number of pts are shown above the respective groups). Experiments were performed in six replicates. White (non-neutralizing) and black solid horizontal bars (neutralizing) indicate mean values of the respective group. t-test or Mann-Whitney Rank Sum test. ** p < 0.01 vs. d1 of the respective cycle.