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. 2018 Sep 20;7(10):294. doi: 10.3390/jcm7100294

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Correlation between pre-treatment NLR in the levels of circulating CD11b⁺CD14^-HLA-DR⁻ cells and ALDH1. (a) Flow cytometric analysis of circulating CD11b⁺CD14^-HLA-DR⁻ cells in isolated peripheral blood mononuclear cells (PBMCs). HLA-DR−CD11b+ cells were gated, and the CD14 negative population was then selected. Representative data from two cancer patients are shown (upper row, the patient with pretreatment NLR < 3; lower row, the patient with pretreatment NLR ≥ 3). Elevated circulating levels of CD11b⁺CD14^-HLA-DR⁻ cells associated with the higher risk of death (b), disease recurrence after treatment (c), and ALDH1 positive staining (d). (e) Positive correlation between the levels of CD11b⁺CD14⁻HLA-DR⁻ cells and pre-treatment NLR in the peripheral circulation.