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. 2018 Oct 12;10(10):382. doi: 10.3390/cancers10100382

Figure 4.

Figure 4

Identification of miR-143 target genes in GBM cells. (A) SYBR Green-based qPCR was performed with total RNA isolated from T98G cells transiently transfected with miR-143-inh or NC-inh. (B) Western blot analysis was performed with protein extracts from miR-143 overexpressed (143-1, 143-2) and EV clones. (C) Western blot analysis of protein extracts from U87 cells treated with 200 nM miR-143-inh or NC-inh, Non-treated cells (NT), and U87 cells with transfection reagent alone (lipo) as loading controls. (D) Dual-luciferase reporter assays were performed where luciferase activity was calculated relative to the NC-inh. (E) IPA analysis showing the interaction of miR-143 with its target genes and SLC30A8 association with glucose metabolism.