Figure 3.

Subcutaneous implantation of the cell-biomaterial system maintains and enhances neural progenitor and neuroepithelium markers expression at 4 weeks. Representative data showing cells within the scaffold expressing the neural progenitor cell marker, nestin, neuroepithelium marker, PAX6, and activated NSC marker, SOX1. (A) Shows qPCR absolute quantification data for PAX6 and nestin reported as log₁₀ the number of gene copies in 100 ng total RNA. There was no significant difference found between regions in PAX6 or nestin expression by ANOVA (p > 0.05) and expressed as mean ± SEM with n = 3. (B) Shows representative image of IHC staining inside the conduits. Arrowheads point to the auto-fluorescent inner border of the chitosan tube surrounding the biomaterial.