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Real-time RT-PCR of lipid mediator biosynthetic enzymes, COX-2 (A), 5-LOX (B), 12-LOX (C), and 12/15-LOX (D) in corneas of PBS-, Tβ4-, ciprofloxacin-, and Tβ4 + ciprofloxacin-treated B6 mice at 3 and 5 days p.i. mRNA expression levels are normalized to β-actin and represented as relative fold change for the gene of interest compared to normal (uninfected) controls ± SEM. N = 8 corneas/group/time point. # P < 0.05 versus PBS; ! P < 0.05 versus ciprofloxacin.
