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. 2018 Oct 5;10(10):370. doi: 10.3390/cancers10100370

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Alkalization of the tumor environment induces a reduction of exosome release. (Left) NTA analysis for the distribution and concentration of the number of exosomes released by the tumor cells cultured in pH conditions ranging from 7.4 to 6.5 (i.e., 7.4, 6.9, 6.7, and 6.5). Data are expressed as means ± SE of three independent experiments. The p-value was <0.0001 in all cellular lines cultured in acidic pH with respect to buffered conditions. (Right) NTA analysis for the distribution and concentration of the number of exosomes released by the tumor cells cultured in pH conditions ranging from 6.5 to 7.4 (i.e., 6.5, 6.7, 6.9, and 7.4). Data are expressed as means ± SE of three independent experiments. The p-value was ≤0.0001 in all cellular lines cultured in buffered conditions with respect to pH 6.5 conditions. * p < 0.1, ** p < 0.01, *** p < 0.001, **** p< 0.0001.